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KMID : 0363720070400030203
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Korean Journal of Anatomy
2007 Volume.40 No. 3 p.203 ~ p.210
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Screening of Interacting Proteins with PV.1 as Downstream Factors of BMP Signal
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Hwang Yoo-Seok
Chae Jeong-Pil
Kim Dong-Sun
Park Kwon-Moo
Bae Yong-Chul
Park Mae-Ja
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Abstract
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Homeodomain transcription factors functioning downstream of BMP ventral pathway have been reported to share similar domain of roles in mesoderm patterning along the dorsal-ventral axis. To elucidate the differential role of PV.1 in the aspect of relationship between dorsal and ventral region, we tried to screen PV.1- interacting proteins. Twenty-four PV.1-interacting proteins were identified by yeast two-hybrid screening. Xvent-2 and Xclaudin-6 among these, went under domain study. The C-terminus of PV.1, more specifically 197-241 region was found to interact with Xclaudin-6. Meanwhile Xvent-2 has mild affinity to overall C-terminal region of PV.1. At the same time it was found that Xvent-2 homodimerizes and also binds to Xclaudin-6.
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KEYWORD
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BMP signal transduction, PV.1 protein, Xvent-2 protein, Xclaudin-6 protein, Yeast two-hybrid, Xenopus laevis
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